A versatile plasmid architecture for mammalian synthetic biology (VAMSyB)

Current molecular cloning strategies generally lack inter-compatibility, are not strictly modular, or applicable to engineer multi-gene expression vectors for transient and stable integration. A standardized platform would advance research, example, by promoting exchange of between groups. Here, we present a versatile plasmid architecture mammalian synthetic biology, which designate VAMSyB, consisting three-tier vector family. Tier-1 is designed easy engineering fusion constructs, as well swapping genes modules tune the functionality vector. Tier-2 expression, constructed directly transferring engineered cassettes from tier-1 vectors. Tier-3 enables integration into host cell through viral transduction, transposons, homology-directed recombination via CRISPR. This VAMSyB expected have broad applicability in field biology. The collection plasmids will be available Addgene.